Module 1: cytoskeleton dynamics
Organisers
A. Abrieu, D. Liakopoulos, L. Blanchoin
The aim of the module is to show the latest developments in the field of cystoskeleton dynamics. The questions raised will explore (i) single molecule microtubule-dependent motility, (ii) the interplay between microtubule and actin dynamics in vitro, and (iii) traction force microscopy in human cells on micropatterns and photoconversion of cellular cytoskeletal components. The students will learn how to pattern microscope slides with special motifs in order to print microtubule or actin and follow their dynamics. They will also learn how to measure single molecule (kinesin) sliding onto microtubules, and how to image and quantify traction forces in human cells. These experiments will be performed using TIRF and widefield microscopy. The training will also include data analysis session.
Lectures
Lectures of the module will be cover several topic and will by given by:
Practical sessions
A. Abrieu, D. Liakopoulos, L. Blanchoin
The aim of the module is to show the latest developments in the field of cystoskeleton dynamics. The questions raised will explore (i) single molecule microtubule-dependent motility, (ii) the interplay between microtubule and actin dynamics in vitro, and (iii) traction force microscopy in human cells on micropatterns and photoconversion of cellular cytoskeletal components. The students will learn how to pattern microscope slides with special motifs in order to print microtubule or actin and follow their dynamics. They will also learn how to measure single molecule (kinesin) sliding onto microtubules, and how to image and quantify traction forces in human cells. These experiments will be performed using TIRF and widefield microscopy. The training will also include data analysis session.
Lectures
Lectures of the module will be cover several topic and will by given by:
- Specific-module lecture 1: Laurent Blanchoin (CEA, Grenoble) – Imaging cytoskeleton dynamics. Tue 19/01 at 11am.
- Specific-module lecture 2: Laetitia Kurzawa (CEA, Grenoble) - Investigating cellular force scaling. Wed 20/01 at 10am.
- Specific-module lecture 3: Sebastian Maurer (CRG, Barcelone) – Microtubule plus end tracking and dynamic instability are mechanistically linked. Wed 20/01 at 11am.
- Specific-module lecture 4: Dimitris Liakopoulos (CRBM, Montpellier) – Analysis of microtubule regulation by kinesin. Thu 21/01 at 11am.
Practical sessions
- Session 1: Simultaneous reconstitution of actin and microtubule dynamics onto micropatterns - TIRF (repeated twice on the 19th and the 21st)
- Session 2: Traction force microscopy (widefield) and Photoconversion (TIRF) (repeated twice on the 20th and the 21st)
- Session 3: Single kinesin motily - TIRF (repeated twice on the 19th and 20th)